The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts

A. Shamay, V. Knopov*, D. Benayahu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10-12-10-6M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD1O/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stroma osteoblast cells.

Original languageEnglish
Pages (from-to)401-405
Number of pages5
JournalCell Biology International
Volume20
Issue number6
DOIs
StatePublished - Jun 1996

Keywords

  • Bone marrow
  • Estrogen receptor
  • Osteoblasts

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