The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts

A. Shamay; V. Knopov; D. Benayahu

doi:10.1006/cbir.1996.0050

The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts

A. Shamay, V. Knopov^*, D. Benayahu

^*Corresponding author for this work

Cell and Developmental Biology

Agricultural Research Organization of Israel

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10^-12-10^-6M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD1O/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stroma osteoblast cells.

Original language	English
Pages (from-to)	401-405
Number of pages	5
Journal	Cell Biology International
Volume	20
Issue number	6
DOIs	https://doi.org/10.1006/cbir.1996.0050
State	Published - Jun 1996

Keywords

Bone marrow
Estrogen receptor
Osteoblasts

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/cbir.1996.0050

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AB - MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10-12-10-6M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD1O/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stroma osteoblast cells.

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The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts

Abstract

Keywords

UN SDGs

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