The etiolated cucumber hypocotyl weight-growth test: A sensitive, easy and ultrafast bioassay for IAA

B. L. Epel, M. Erlanger, A. Yahalom

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive, easy and fast bioassay is described for the detection of IAA. The bioassay consists of measuring the weight increase of hypocotyl sections from etiolated cucumber seedlings incubated in a simple medium containing 2 mM KCl, 0.1 mM CaCl and 10 mg/l chloramphenicol (pH 7). The sensitivity of the test is comparable to that of the Avena first internode test, exhibiting a significant response at an IAA concentration as low as 1 ng/ml. The bioassay requires only 3 h, and is easy to perform. The slope of the log-linear concentration-response curve is moderately steep giving good accuracy. The assay is insensitive to the pH of the media between 4 to 7 and can be performed in the presence or absence of buffer. The growth of the etiolated hypocotyl sections is insensitive to GA and to kinetin except at high concentrations.

Original languageEnglish
Pages (from-to)3-14
Number of pages12
JournalPlant Growth Regulation
Volume5
Issue number1
DOIs
StatePublished - Jan 1987

Keywords

  • IAA
  • auxin-bioassay
  • cucumber
  • hypocotyl elongation

Fingerprint

Dive into the research topics of 'The etiolated cucumber hypocotyl weight-growth test: A sensitive, easy and ultrafast bioassay for IAA'. Together they form a unique fingerprint.

Cite this