The establishment and characterization of a new balb/c angiosarcoma tumor system

Isabel Zvibel*, Avraham Raz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


A BALB‐3T3/A31 untransformed cloned cell line and 3 selected variants derived from this parental cell line, expressing 3 increasingly malignant phenotypes, have been established and characterized in vivo and in culture. This new tumor series, identified as angiosarcoma, consists of an anchorage‐independent nontumorigenic cell clone and 2 sublines exhibiting tumorigenic and metastatic properties. Morphological examination revealed that the 3 transformed cell variants differed from the normal parental cells and were not contact‐inhibited. Karyotype and rate of cell proliferation in culture were similar for all the cell variants. Cytoskeletal visualization by immunofluorescence staining revealed that the tumorigenic and metastatic cell lines expressed an altered organization of actin cables and a smaller number of vinculin‐containing focal contacts. Lactoperoxidase iodination of cell surface proteins showed the appearance of an Mγ 86,000 protein in the tumorigenic and metastatic cell variants. Analysis of cell‐surface glycoproteins demonstrated an increased sialylation of Mγ 66,000 and Mγ 62,000 glycoproteins in the transformed, tumorigenic and metastatic cell lines. This angiosarcoma tumor model system allows investigation of cellular characteristics which might be relevant to specific stages in tumor progression.

Original languageEnglish
Pages (from-to)261-272
Number of pages12
JournalInternational Journal of Cancer
Issue number2
StatePublished - 15 Aug 1985
Externally publishedYes


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