The epitranscriptome toolbox

Sharon Moshitch-Moshkovitz, Dan Dominissini, Gideon Rechavi*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

39 Scopus citations

Abstract

In the last decade, the notion that mRNA modifications are involved in regulation of gene expression was demonstrated in thousands of studies. To date, new technologies and methods allow accurate identification, transcriptome-wide mapping, and functional characterization of a growing number of RNA modifications, providing important insights into the biology of these marks. Most of the methods and approaches were developed for studying m6A, the most prevalent internal mRNA modification. However, unique properties of other RNA modifications stimulated the development of additional approaches. In this technical primer, we will discuss the available tools and approaches for detecting and studying different RNA modifications.

Original languageEnglish
Pages (from-to)764-776
Number of pages13
JournalCell
Volume185
Issue number5
DOIs
StatePublished - 3 Mar 2022

Funding

FundersFunder number
DIP
German-Israeli Project Cooperation
Varda and Boaz Dotan Research Center in Hemato-Oncology
Flight Attendant Medical Research Institute
Horizon 2020 Framework Programme
European Research Council
Human Frontier Science ProgramCDA 00048/2018
Bundesministerium für Bildung und Forschung
Israel Science Foundation2494/18, 2625/17
Tel Aviv University
Horizon 2020743168

    Keywords

    • RNA modifications
    • epitranscriptomics
    • mass spectrometry
    • transcriptome

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