TY - JOUR
T1 - The effect of relaxin and prostaglandin E2 on the motility of human spermatozoa
AU - Colon, J. M.
AU - Ginsburg, F.
AU - Lessing, J. B.
AU - Schoenfeld, C.
AU - Goldsmith, L. T.
AU - Amelar, R. D.
AU - Dubin, L.
AU - Weiss, G.
N1 - Funding Information:
Received May 5, 1986; revised and accepted August 13, 1986. *Supported by grant HD-12395 from the National Institutes of Health and by a grant from the Mellon Foundation. tPresented in part at the Forty-First Annual Meeting of The American Fertility Society, Chicago, Illinois, September 28 to October 2, 1985. :j:Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey. §Reprint requests: Jose M. Colon, M.D., Department of Obstetrics and Gynecology, UMDNJ-New Jersey Medical School, 100 Bergen Street, Medical Science Building, E506, Newark, New Jersey 07103. "Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, New York University School of Medicine. Present address: Department of Obstetrics and Gynecology A, Tel-Aviv Medical Center, Serlin Hospital, Sackler School of Medicine, Tel-Aviv, Israel. ~Department of Urology, New York University School of Medicine and Fertility Laboratory, Inc.
PY - 1986
Y1 - 1986
N2 - Relaxin and prostaglandin E2 (PGE2) are present in human semen and have been shown to affect sperm motility. The authors further examined the effects of porcine relaxin and PGE2 on the motility of human spermatozoa. A dose-response study revealed that PGE2 at a concentration of 25 μg/ml is most effective in improving the motility of washed human sperm. Relaxin (100 ng/ml), PGE2 (25 μg/ml), or the two combined have no effect on the motility of spermatozoa in fresh, normal semen, suggesting that the constituents of fresh semen are optimal for motility. Relaxin and PGE2 individually improve the motility of washed spermatozoa. However, relaxin, but not PGE2, improves the motility of sperm in semen incubated at 37°C for 5 hours (aged). In contrast to the individual substances, a combination of relaxin + PGE2 has no effect on the motility of washed spermatozoa or aged spermatozoa, suggesting that these two substances antagonize each other's action on sperm motility. The presence of both relaxin and PGE2 in seminal plasma with normal motility spermatozoa suggests that other factors in seminal plasma regulate the effects of these substances on sperm motility.
AB - Relaxin and prostaglandin E2 (PGE2) are present in human semen and have been shown to affect sperm motility. The authors further examined the effects of porcine relaxin and PGE2 on the motility of human spermatozoa. A dose-response study revealed that PGE2 at a concentration of 25 μg/ml is most effective in improving the motility of washed human sperm. Relaxin (100 ng/ml), PGE2 (25 μg/ml), or the two combined have no effect on the motility of spermatozoa in fresh, normal semen, suggesting that the constituents of fresh semen are optimal for motility. Relaxin and PGE2 individually improve the motility of washed spermatozoa. However, relaxin, but not PGE2, improves the motility of sperm in semen incubated at 37°C for 5 hours (aged). In contrast to the individual substances, a combination of relaxin + PGE2 has no effect on the motility of washed spermatozoa or aged spermatozoa, suggesting that these two substances antagonize each other's action on sperm motility. The presence of both relaxin and PGE2 in seminal plasma with normal motility spermatozoa suggests that other factors in seminal plasma regulate the effects of these substances on sperm motility.
UR - http://www.scopus.com/inward/record.url?scp=0023010525&partnerID=8YFLogxK
U2 - 10.1016/s0015-0282(16)49894-8
DO - 10.1016/s0015-0282(16)49894-8
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AN - SCOPUS:0023010525
SN - 0015-0282
VL - 46
SP - 1133
EP - 1139
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -