TY - JOUR
T1 - The effect of pressure on the excited-state proton transfer in the wild-type green fluorescent protein
AU - Leiderman, Pavel
AU - Huppert, Dan
AU - Remington, S. James
AU - Tolbert, Laren M.
AU - Solntsev, Kyril M.
N1 - Funding Information:
This work was supported by grants from the Binational US-Israel Science Foundation (for D.H., S.J.R., and L.M.T.), the US National Science Foundation (CHE-0456892 for L.M.T. and K.M.S.), and the James-Franck German–Israel Program in Laser-Matter Interactions for D.H.
PY - 2008/4/10
Y1 - 2008/4/10
N2 - Ultrahigh-pressure dependence of the excited-state proton transfer (ESPT) in the wild-type green fluorescence protein (wt-GFP) in D2O was measured using steady-state and picosecond time-resolved fluorescence spectroscopies. The proton dissociation rate of the chromophore is almost insensitive to a pressure increase up to about 1.1 GPa. In contrast, the diffusion-limited geminate recombination kinetics is strongly affected by pressure, decreasing the effective dimensionality of proton diffusion as pressure increased. The GFP β-barrel structure sustains the high pressure and unfolds only at P > 1.5 GPa.
AB - Ultrahigh-pressure dependence of the excited-state proton transfer (ESPT) in the wild-type green fluorescence protein (wt-GFP) in D2O was measured using steady-state and picosecond time-resolved fluorescence spectroscopies. The proton dissociation rate of the chromophore is almost insensitive to a pressure increase up to about 1.1 GPa. In contrast, the diffusion-limited geminate recombination kinetics is strongly affected by pressure, decreasing the effective dimensionality of proton diffusion as pressure increased. The GFP β-barrel structure sustains the high pressure and unfolds only at P > 1.5 GPa.
UR - http://www.scopus.com/inward/record.url?scp=41349113756&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2008.02.079
DO - 10.1016/j.cplett.2008.02.079
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AN - SCOPUS:41349113756
SN - 0009-2614
VL - 455
SP - 303
EP - 306
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 4-6
ER -