TY - JOUR
T1 - The effect of plasma depletion of platelet concentrates on platelet aggregation and phosphatidylserine expression
AU - Zilber, Moshe
AU - Friedman, Ziva
AU - Shapiro, Hava
AU - Shapira, Shirley
AU - Radnay, Judith
AU - Ellis, Martin H.
PY - 2003/1
Y1 - 2003/1
N2 - Plasma-reduced platelet concentrates are commonly administered to prevent febrile transfusion reactions and to avoid fluid overload in neonates. Because little is known about the influence of centrifugation and resuspension on functional aspects of platelets, we examined the effects of plasma-reduction on platelet aggregation and platelet-dependent thrombin generation. Our results show that plasma reduction and resuspension of the platelet pellet in saline or plasma results in a significant reduction in platelet aggregation to a combination of the platelet agonists adenosine diphosphate and epinephrine (p < 0.001). In contrast, when a combination of the more potent agonists collagen and thrombin was used, platelet aggregation was maintained. Likewise, no decline was observed in platelet-dependent thrombin generation as measured by the functional prothrombinase assay or Annexin V binding. We conclude that centrifugation and resuspension of platelets to render the concentrate plasma-free, as a routine procedure in blood banking, variably affects in vitro platelet aggregability but does not significantly affect platelet-dependent thrombin generation.
AB - Plasma-reduced platelet concentrates are commonly administered to prevent febrile transfusion reactions and to avoid fluid overload in neonates. Because little is known about the influence of centrifugation and resuspension on functional aspects of platelets, we examined the effects of plasma-reduction on platelet aggregation and platelet-dependent thrombin generation. Our results show that plasma reduction and resuspension of the platelet pellet in saline or plasma results in a significant reduction in platelet aggregation to a combination of the platelet agonists adenosine diphosphate and epinephrine (p < 0.001). In contrast, when a combination of the more potent agonists collagen and thrombin was used, platelet aggregation was maintained. Likewise, no decline was observed in platelet-dependent thrombin generation as measured by the functional prothrombinase assay or Annexin V binding. We conclude that centrifugation and resuspension of platelets to render the concentrate plasma-free, as a routine procedure in blood banking, variably affects in vitro platelet aggregability but does not significantly affect platelet-dependent thrombin generation.
KW - Aggregation
KW - Depletion
KW - Phosphatidylserine expression
KW - Plasma
KW - Platelet concentrates
KW - Prothrombinase
UR - http://www.scopus.com/inward/record.url?scp=0037222615&partnerID=8YFLogxK
U2 - 10.1177/107602960300900105
DO - 10.1177/107602960300900105
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C2 - 12643322
AN - SCOPUS:0037222615
VL - 9
SP - 39
EP - 44
JO - Clinical and Applied Thrombosis/Hemostasis
JF - Clinical and Applied Thrombosis/Hemostasis
SN - 1076-0296
IS - 1
ER -