The effect of magnesium sulfate on the placental corticotropin-releasing factor (CRF) and CRF binding protein mRNA expression in perfused human placental cotyledon

Nesia Kropach, Dorin Holtzman, Dana Vitner, Jacob Bar, Alon Chen, Michal Kovo

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Stress stimuli and inflammation influence the secretion of the placental corticotropin-releasing factor CRF (CRF) that has a significant role in controlling the timing of birth. The CRF-binding protein (CRF-BP) binds CRF with high affinity and inhibits its activity. Magnesium sulfate (MgSO4) has been known to ameliorate maternal, fetal and gestational tissue-associated inflammatory response. We aimed to study the effect of MgSO4 on the CRF and CRF-BP mRNA expression levels in perfused human cotyledon.Methods: Placentas from elective caesarean section were obtained and selected cotyledons were cannulated and dually perfused ex-vivo within 30 min. MgSO4 (7 mg/dl) was added to the maternal reservoir. Each perfusion experiment was conducted for 180 min. At the end of the experiment, RNA was extracted from the perfused cotyledon, and RT-PCR was performed to quantify the expression of CRF and CRF-BP. Human HPRT gene served as a reference gene.Results: Perfusion with MgSO4 (n = 3) induced a significantly lower CRF and higher CRF-BP mRNA expression compared to placentas perfused only with medium (n = 3).Conclusion: In the human placenta, MgSO4 possibly exerts its action through different modulation on the CRF and CRF-BP expression.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume29
Issue number3
DOIs
StatePublished - 1 Feb 2016
Externally publishedYes

Keywords

  • Corticotropin-releasing factor
  • magnesium sulfate
  • placenta

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