TY - JOUR
T1 - The effect of LH on the fertilizability and developmental capacity of rat oocytes matured in vitro.
AU - Shalgi, R.
AU - Dekel, N.
AU - Kraicer, P. F.
PY - 1979/3
Y1 - 1979/3
N2 - The effect of adding LH (10 microgram NIH-LH-B8/ml) to the medium in which oocytes were undergoing maturation in vitro was studied. The fertilizability of the oocytes was evaluated in the sterile oviduct of a unilaterally ovariectomized, mated recipient. Freshly ovulated oocytes, used as a control of the method, were fertilized at a rate of 72%. Only 14% of oocytes matured in culture (without LH) were penetrated by spermatozoa, and 11% were fertilized normally. Addition of LH to the medium increased these proportions to 43 and 33% respectively. Oocytes matured in the presence of LH were able to develop into apparently normal rats. It is concluded that, although oocytes can mature in vitro spontaneously, and that these matured oocytes can be fertilized, addition of LH increases the numbers 3-fold. LH therefore has a direct maturation-promoting action on the rat oocyte-cumulus complex in vitro.
AB - The effect of adding LH (10 microgram NIH-LH-B8/ml) to the medium in which oocytes were undergoing maturation in vitro was studied. The fertilizability of the oocytes was evaluated in the sterile oviduct of a unilaterally ovariectomized, mated recipient. Freshly ovulated oocytes, used as a control of the method, were fertilized at a rate of 72%. Only 14% of oocytes matured in culture (without LH) were penetrated by spermatozoa, and 11% were fertilized normally. Addition of LH to the medium increased these proportions to 43 and 33% respectively. Oocytes matured in the presence of LH were able to develop into apparently normal rats. It is concluded that, although oocytes can mature in vitro spontaneously, and that these matured oocytes can be fertilized, addition of LH increases the numbers 3-fold. LH therefore has a direct maturation-promoting action on the rat oocyte-cumulus complex in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0018451565&partnerID=8YFLogxK
U2 - 10.1530/jrf.0.0550429
DO - 10.1530/jrf.0.0550429
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C2 - 439077
AN - SCOPUS:0018451565
SN - 0022-4251
VL - 55
SP - 429
EP - 435
JO - Journal of Reproduction and Fertility
JF - Journal of Reproduction and Fertility
IS - 2
ER -