The effect of interferon-γ and tumor necrosis factor-α on the expression of ICAM-1 and HLA-DR molecules on cells of a human germ cell neoplasm and their susceptibility to lysis by lymphokine-activated killer cells

Avi Eisenthal, Oleg Marder, Batya Maymon, Faina Misonzhnik, Yehuda Skornick, Eli Brazowski, Bernard Czernobilsky, Heinrich Walt, Beatriz Lifschitz-Mercer

Research output: Contribution to journalArticlepeer-review

Abstract

The ICAM-1 molecule plays a role in the interaction of NK cells with a variety of tumor cells, including carcinoma, melanoma and glioblastoma cells. In the present study, we analyzed the effect of IFN-γ and TNF-α on both the expression of HLA-DR and ICAM-1 molecules on HGCN (Germa-2), and on their susceptibility to lysis by LAK cells. Our results show that 1000 U/ml IFN-γ induced a substantial increase in the expression of both ICAM-1 molecules and HLA-DR on the cell surface, while the effect of TNF-α on the expression of these molecules was substantially less prominent. When Germa-2 cells, previously exposed to 1000 U/ml IFN-γ, were employed as target cells in a 4-hour 51Cr release assay, a statistically significant increase in the lysis by LAK cells was noted. These results show that in the presence of IFN-γ, Germa-2 tumor cells undergo modulation which affects both the expression of ICAM-1 and HLA-DR molecules as well as their susceptibility to lysis by LAK cells.

Original languageEnglish
Pages (from-to)205-208
Number of pages4
JournalPathobiology
Volume66
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Cytokines
  • Cytotoxicity
  • Germ cell tumor, human
  • ICAM-1
  • LAK cells

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