The effect of heat treatment on the damage and recovery of the protein synthesis mechanism of human kidney cell line

Ilan Bleiberg, Ezra Sohar

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of supraoptimal temperature on the suppression and recovery of protein synthesis activity in cultures of human kidney cell line was studied. It was shown that 44°C is a critical temperature to these cells, and after exposure to this temperature for 3 hours no recovery of protein synthesis activity could be detected. The cloning efficiency of cells exposed to 44°C for 2 hours was irreversibly reduced to zero. Protein synthesis at 43°C was much less affected, showing considerable recovery after eight hours exposure. Differences in heat susceptibility of individual cells in the culture were observed, but the reason for this heterogeneity is not yet known.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume17
Issue number1
DOIs
StatePublished - Dec 1975

Keywords

  • Clones
  • Heat
  • Human Kidney Cell Line
  • Protein Synthesis

Fingerprint

Dive into the research topics of 'The effect of heat treatment on the damage and recovery of the protein synthesis mechanism of human kidney cell line'. Together they form a unique fingerprint.

Cite this