The effect of supraoptimal temperature on the suppression and recovery of protein synthesis activity in cultures of human kidney cell line was studied. It was shown that 44°C is a critical temperature to these cells, and after exposure to this temperature for 3 hours no recovery of protein synthesis activity could be detected. The cloning efficiency of cells exposed to 44°C for 2 hours was irreversibly reduced to zero. Protein synthesis at 43°C was much less affected, showing considerable recovery after eight hours exposure. Differences in heat susceptibility of individual cells in the culture were observed, but the reason for this heterogeneity is not yet known.
|Number of pages||10|
|Journal||Virchows Archiv B Cell Pathology Including Molecular Pathology|
|State||Published - Dec 1975|
- Human Kidney Cell Line
- Protein Synthesis