The effect of electrostatic shielding on H tunneling in R67 dihydrofolate reductase

Atsushi Yahashiri, Guy Nimrod, Nir Ben-Tal, Elizabeth E. Howell, Amnon Kohen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

(Figure Presented) The nature of the C-H→C transfer catalyzed by the primitive plasmidal enzyme R67 dihydrofolate reductase was examined as function of the ionic strength of the medium. Kinetic isotope effects and theoretical calculations suggested that, at high ionic strength, electrostatic repulsion between the H donor and H acceptor is reduced, and the reaction coordinate is better organized for H tunneling.

Original languageEnglish
Pages (from-to)2620-2623
Number of pages4
JournalChemBioChem
Volume10
Issue number16
DOIs
StatePublished - 2 Nov 2009

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM065368

    Keywords

    • Dihydrofolate reductase
    • Ionic strength
    • Isotope effects
    • Marcus theory
    • Tunneling

    Fingerprint

    Dive into the research topics of 'The effect of electrostatic shielding on H tunneling in R67 dihydrofolate reductase'. Together they form a unique fingerprint.

    Cite this