The effect of decalcification on the microstructure of articular cartilage assessed by 2H double quantum filtered spectroscopic MRI

Keren Keinan-Adamsky, Hadassah Shinar, Gil Navon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

2H quadrupolar splitting of deuterated water molecules is a sensitive measure of the order and density of the collagen fibers in articular cartilage. In the calcified zone, near the bone, two pairs of quadrupolar split satellites were previously observed. To examine whether the large splitting observed originates from the presence of calcium ions and hydroxyapatite, one-dimensional 2H single and double quantum filtered spectroscopic imaging were performed on articular cartilage-bone plugs before and after decalcification. After decalcification, the magnitude splitting of the two pairs of satellites did not change and orientation dependency was kept. However, the intensity of the large splitting was greatly enhanced. According to these results the two pairs of satellites do not stem from the presence of calcium ions and hydroxyapatite but originate from the presence of two groups of collagen fibers with different degrees of hydration. The enhanced intensity of the large splitting is attributed to an increased amount of water molecules that fill the void, resulting from the removal of hydroxyapatite, which resides near the fibers responsible for the large splitting. The quadrupolar splitting observed in the trabecular bone was not orientation-dependent, indicating a random orientation of the collagen fibers in that tissue.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalMagnetic Resonance Materials in Physics, Biology, and Medicine
Volume18
Issue number5
DOIs
StatePublished - Oct 2005

Funding

FundersFunder number
Israel Academy of Sciences and Humanities

    Keywords

    • Articular cartilage
    • Bone
    • Collagen fibers
    • Decalcification
    • H DQF spectroscopic MRI

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