The effect of centrifugal force on mRNA levels of collagenase, collagen type-I, tissue inhibitors of metalloproteinases and β-actin in cultured human periodontal ligament fibroblasts

M. Redlich, H. Roos, E. Reichenberg, B. Zaks, A. Grosskop, I. Bar Kana, S. Pitaru, A. Palmon

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The aim of orthodontic treatment is to relocate teeth abnormally positioned in the jaws. This is achieved by application of continuous force on the tooth, which is immediately being sensed by the periodontal ligament (PDL), bone and the gingiva. Since the bony response is mediated by the PDL, tooth movement is primarily a PDL phenomenon. Objectives: Thus, the purpose of the present study was to evaluate the direct effect of force (excluding the in vivo tissue response) on the molecular level of matrix metalloproteinase-1 (MMP-1) and collagen type-I (Col-I) in human PDL fibroblasts. Methods: PDL cell culture flasks were centrifuged for 10, 20, 30, 60, 90 and 120 min by horizontal microplate rotor. The effect of force on mRNA levels of β-actin, MMP-1, Col-I, tissue inhibitors-1 and -2 (TIMPs) genes was analyzed by RT-PCR. Results: The results showed that force had no effect on the mRNA levels of β-actin during the first 90 min of application of force, indicating for the first time the use of β-actin gene as an internal invariant control. It increased the mRNA levels of MMP-1 while almost no effect on Col-I and TIMPs was observed. Conclusions: The results indicate that PDL remodeling following application of orthodontic force could be partly attributed to the direct effect of the force on MMP-1 gene expression in fibroblasts.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalJournal of Periodontal Research
Volume39
Issue number1
DOIs
StatePublished - Feb 2004

Keywords

  • Gene expression
  • Matrix metalloproteinase-1
  • Orthodontic force
  • Periodontal ligament

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