TY - JOUR
T1 - The effect of brief exercise on circulating CD34+ stem cells in early and late pubertal boys
AU - Zaldivar, Frank
AU - Eliakim, Alon
AU - Radom-Aizik, Shlomit
AU - Leu, Szu Yun
AU - Cooper, Dan M.
PY - 2007/4
Y1 - 2007/4
N2 - We tested the hypothesis that exercise could stimulate CD34 peripheral blood hematopoietic stem cells (PBSC) in children. Fourteen early pubertal boys (EP, age 10.3 ± 0.3 y) and 13 late pubertal boys (LP, age 16.5 ± 0.4 y) performed 20 min of moderate-to-vigorous cycle ergometer exercise. Blood was drawn before and after exercise. Cells were stained for surface CD34. Plasma granulocyte colony stimulating factor (G-CSF), Fms-like tyrosine kinase-3 (FLT-3), and stromal cell-derived factor-1 (SDF-1) were measured using ELISA. Exercise substantially increased PBSC (in EP from 112 ± 21 to 182 ± 30 cells/μL, p = 0.0007; in LP from 63 ± 8 to 152 ± 21, p = 0.0008), and to a smaller extent FLT-3 (in EP from 98 ± 5 to 110 ± 6 pg/mL, p < 0.0001; in LP from 73 ± 6 to 92 ± 6, p < 0.0001) and G-CSF (in EP from 26 ± 4 to 29 ± 4 pg/mL, p < 0.0001; in LP from 14 ± 1 to 18 ± 1, p < 0.0001). Baseline levels of PBSC, FLT-3, and G-CSF were significantly higher in EP. Exercise increased SDF-1α only in LP, and the FLT-3 increase was greater in LP than EP. Brief exercise affects PBSC and PBSC mediators in children.
AB - We tested the hypothesis that exercise could stimulate CD34 peripheral blood hematopoietic stem cells (PBSC) in children. Fourteen early pubertal boys (EP, age 10.3 ± 0.3 y) and 13 late pubertal boys (LP, age 16.5 ± 0.4 y) performed 20 min of moderate-to-vigorous cycle ergometer exercise. Blood was drawn before and after exercise. Cells were stained for surface CD34. Plasma granulocyte colony stimulating factor (G-CSF), Fms-like tyrosine kinase-3 (FLT-3), and stromal cell-derived factor-1 (SDF-1) were measured using ELISA. Exercise substantially increased PBSC (in EP from 112 ± 21 to 182 ± 30 cells/μL, p = 0.0007; in LP from 63 ± 8 to 152 ± 21, p = 0.0008), and to a smaller extent FLT-3 (in EP from 98 ± 5 to 110 ± 6 pg/mL, p < 0.0001; in LP from 73 ± 6 to 92 ± 6, p < 0.0001) and G-CSF (in EP from 26 ± 4 to 29 ± 4 pg/mL, p < 0.0001; in LP from 14 ± 1 to 18 ± 1, p < 0.0001). Baseline levels of PBSC, FLT-3, and G-CSF were significantly higher in EP. Exercise increased SDF-1α only in LP, and the FLT-3 increase was greater in LP than EP. Brief exercise affects PBSC and PBSC mediators in children.
UR - http://www.scopus.com/inward/record.url?scp=34147128693&partnerID=8YFLogxK
U2 - 10.1203/pdr.0b013e3180332d36
DO - 10.1203/pdr.0b013e3180332d36
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C2 - 17515877
AN - SCOPUS:34147128693
SN - 0031-3998
VL - 61
SP - 491
EP - 495
JO - Pediatric Research
JF - Pediatric Research
IS - 4
ER -