The effect of angiotensin II on myosin heavy chain expression in cultured myocardial cells

Noa Shalitin, Menahem Friedman, Hadassa Schlesinger, Yael Barhum, Maurice J. Levy, Wolfgang Schaper, Gania Kessler-Icekson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Angiotensin II (AII), the principal mediator of the renin-angiotensin system, is an important regulator of vascular and cardiac homeostasis. All has also been shown to be a regulator of cardiac hypertrophy and of the corresponding changes in amount and composition of certain tissue proteins. We examined the trophic effects of AII on cultured myocytes derived from neonatal rat ventricles and followed, by Northern blot analysis and polyacrylamide gel electrophoresis, the expression of α- and β-myosin heavy chain iso-mRNAs and isoproteins. Our findings show that a single administration of AII is sufficient to induce a trophic response in cultured beating myocytes and to enhance the expression of β-myosin heavy chain iso- mRNA and isoprotein, having no effect on α-myosin heavy chain. Induction of α-myosin heavy chain expression by thyroid hormone before AII was administered showed that AII could not potentiate a shift from α- to β- myosin heavy chain predominance. We suggest that the potency of AII to regulate the expression of myosin heavy chain isogenes is restricted to the β isoform and is overridden by thyroid hormone.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume32
Issue number9
DOIs
StatePublished - Oct 1996
Externally publishedYes

Keywords

  • angiotensin II
  • cellular hypertrophy
  • myosin heavy chains
  • neonatal heart myocytes

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