TY - JOUR
T1 - The effect of alpha-fetoprotein on the growth of placental cells in vitro
AU - Toder, V.
AU - Blank, M.
AU - Gold-Gefter, L.
AU - Nebel, L.
N1 - Funding Information:
The authors wish to express their gratitude to Professor E. Zimmerman, Children's Hospital, Research Foundation, Cincinnati, OH, USA, for the anti-AFP serum used in this experiment. Thanks are due to Dr A. Benenson and Mrs V. Eyal for their contribution to the isolation of mouse AFP. This work was supported by a grant from the Ministry of Health, Israel.
PY - 1983
Y1 - 1983
N2 - Cell cultures of early mouse placentae were studied in the presence of mouse amniotic fluid or isolated alpha-fetoprotein (AFP). Four cell types were observed: polygonal cells, giant cells, small round cells and fibroblasts. The number of polygonal cells increased in placental cultures in the presence of amniofic fluid or AFP, suggesting de novo formation and proliferation in vitro. These preparations were found to stimulate the DNA synthesis in polygonal cells, as demonstrated by 3H-thymidine labelling experiments. Polygonal cells also show a significant increase in 3H-leucine incorporation, indicating more active protein synthesis under the effect of amniotic fluid or AFP. These data suggest that AFP may be one of the fetal factors promoting trophoblastic differentiation.
AB - Cell cultures of early mouse placentae were studied in the presence of mouse amniotic fluid or isolated alpha-fetoprotein (AFP). Four cell types were observed: polygonal cells, giant cells, small round cells and fibroblasts. The number of polygonal cells increased in placental cultures in the presence of amniofic fluid or AFP, suggesting de novo formation and proliferation in vitro. These preparations were found to stimulate the DNA synthesis in polygonal cells, as demonstrated by 3H-thymidine labelling experiments. Polygonal cells also show a significant increase in 3H-leucine incorporation, indicating more active protein synthesis under the effect of amniotic fluid or AFP. These data suggest that AFP may be one of the fetal factors promoting trophoblastic differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0020526488&partnerID=8YFLogxK
U2 - 10.1016/S0143-4004(83)80019-8
DO - 10.1016/S0143-4004(83)80019-8
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AN - SCOPUS:0020526488
VL - 4
SP - 79
EP - 85
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 1
ER -