The effect of agmatine on ischemic and nonischemic isolated rat heart

S. Greenberg, A. Finkelstein, J. Gurevich, E. Brazowski, F. Rosenfeld, I. Shapira, J. George, S. Laniado, G. Keren

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The natural polyamines play a protective role during ischemic injury. We studied the effects of agmatine on ischemic and nonischemic isolated rat hearts. Methods: Thirty-one rats were randomly assigned to one of four experimental groups. Sixteen rats were injected with saline (group 1, n = 9; group 3, n = 7), and 15 rats were injected with 100 mg/kg of agmatine (group 2, n = 8; group 4, n = 7). Injections were given twice: 24 hours and I hour before the experiment. Using the modified Langendorf model, rat hearts were perfused with Krebs-Henseleit solution for 105 minutes during phase 1 of the experiment (groups 1 and 2). During phase 2, hearts were exposed to 45 minutes of global ischemia (groups 3 and 4). Results: During phase 1, no statistically significant differences were observed between the agmatine and the control groups. During phase 2, agmatine caused a significant increase in left ventricular pressure (P < .003). At the end of reperfusion, P(max) was 11.1% ± 10% from the baseline levels versus only 82% ± 5% in the control group. After 20 minutes of reperfusion, dP/dt (first-time derivative of the ventricular pressure) in the agmatine group reached full recovery of 106% ± 12% versus only 64% ± 14% in the saline group (P = .059). Agmatine also caused a significant increase in coronary flow rate (P < .004) throughout the reperfusion period. Quantitative immunohistochemical staining disclosed reduced cell damage in the agmatine-treated hearts (P < .02) versus the control group. Conclusion: Agmatine injection given before induced ischemia improves hemodynamic recovery by mechanisms that may be attributed to its vasodilatory properties.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalJournal of Cardiovascular Pharmacology and Therapeutics
Volume4
Issue number3
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Agmatine
  • Ischemia
  • Isolated heart
  • Polyamines

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