TY - JOUR
T1 - The effect of 2-mercaptoacetyl-l-phenylalanyl-l-leucine, a specific inhibitor of pseudomonas aeruginosa elastase, on experimental pseudomonas keratitis in rabbit eyes
AU - Spierer, Abraham
AU - Kessler, Efrat
N1 - Funding Information:
ACKNOWLEDGEMENTS This study was supported by grants from the Recanati Fund for Medical Research and the Pinhas Sapir Fund, Mifal Hapais (E.K.). We thank Dr. Shmaryahu Blumberg of the Weizmann Institute of Science for synthesizing and providing us with the inhibitor HS-Ac-Phe-Leu and for helpful discussions in the course of this study. We also thank Dr. Michaela Modan for performing the s t a t i s t i c a l analysis and M r s . Mary Safrin for excellent technical assistance.
PY - 1984
Y1 - 1984
N2 - The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatment in experimentally induced Paeudomonas keratitis in rabbits. Twenty eight hours after infection, the eyes that received both, gentamicin and HS-Ac-Phe-Leu showed significantly less corneal melting than those treated with the antibiotics alone, demonstrating the beneficial effect of the inhibitor. Forty eight hours after infection, the difference in clinical appearance between the two treatment groups diminished and was statistically insignificant. No adverse effects were noted in the eyes that received the inhibitor in spite of its frequent application. The protective effect of HS-Ac-Phe-Leu, seen on the first day of the experiment suggests further exploration of the therapeutic potential of this or other, similarly structured, specific and potent inhibitors of Pseudomonas elastase.
AB - The compound 2-mercaptoacetyl-L-phenylalanyl-L-leucine (HS-Ac-Phe-Leu) is a potent and specific inhibitor of Pseudomonas aeruginosa elastase, effectively inhibiting the elastase activity both, in vitro and within the rabbit cornea. This inhibitor was therefore evaluated as an adjunct to antibiotics (gentamicin) treatment in experimentally induced Paeudomonas keratitis in rabbits. Twenty eight hours after infection, the eyes that received both, gentamicin and HS-Ac-Phe-Leu showed significantly less corneal melting than those treated with the antibiotics alone, demonstrating the beneficial effect of the inhibitor. Forty eight hours after infection, the difference in clinical appearance between the two treatment groups diminished and was statistically insignificant. No adverse effects were noted in the eyes that received the inhibitor in spite of its frequent application. The protective effect of HS-Ac-Phe-Leu, seen on the first day of the experiment suggests further exploration of the therapeutic potential of this or other, similarly structured, specific and potent inhibitors of Pseudomonas elastase.
UR - http://www.scopus.com/inward/record.url?scp=0021359976&partnerID=8YFLogxK
U2 - 10.3109/02713688409003066
DO - 10.3109/02713688409003066
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 6562000
AN - SCOPUS:0021359976
SN - 0271-3683
VL - 3
SP - 645
EP - 650
JO - Current Eye Research
JF - Current Eye Research
IS - 4
ER -