The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide

Nathan W. Luedtke; Judy S. Hwang; Eileen Nava; Dalia Gut; Moshe Kol; Yitzhak Tor

doi:10.1093/nar/gkg758

The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide

Nathan W. Luedtke
, Judy S. Hwang
, Eileen Nava
, Dalia Gut
, Moshe Kol
, Yitzhak Tor^*

^*Corresponding author for this work

School of Chemistry

Department of Chemistry/Biochemistry
University of California at San Diego
Yale University
School of Chemistry
Tel Aviv University
Department of Chemistry/Biochemistry

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNA^Phe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)₂eilatin]²⁺ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (K_d ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.

Original language	English
Pages (from-to)	5732-5740
Number of pages	9
Journal	Nucleic Acids Research
Volume	31
Issue number	19
DOIs	https://doi.org/10.1093/nar/gkg758
State	Published - 1 Oct 2003

Funding

Funders	Funder number
Beckman Scholars Program
National Institutes of Health	AI 47673
National Institute of General Medical Sciences	R01GM058447
Israel Academy of Sciences and Humanities
Israel Science Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1093/nar/gkg758

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title = "The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide",

abstract = "Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.",

author = "Luedtke, \{Nathan W.\} and Hwang, \{Judy S.\} and Eileen Nava and Dalia Gut and Moshe Kol and Yitzhak Tor",

note = "Funding Information: Financial support was provided, in part, by the National Institutes of Health (AI 47673 and GM 58447 to Y.T.). D.G. and M.K. are grateful to the Israel Science Foundation and the Israel Academy of Sciences and Humanities for support. J.S.H. thanks the Beckman Scholars Program for an undergraduate research fellowship.",

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AU - Luedtke, Nathan W.

AU - Hwang, Judy S.

AU - Nava, Eileen

AU - Gut, Dalia

AU - Kol, Moshe

AU - Tor, Yitzhak

N1 - Funding Information: Financial support was provided, in part, by the National Institutes of Health (AI 47673 and GM 58447 to Y.T.). D.G. and M.K. are grateful to the Israel Science Foundation and the Israel Academy of Sciences and Humanities for support. J.S.H. thanks the Beckman Scholars Program for an undergraduate research fellowship.

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N2 - Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.

AB - Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.

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The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide

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