The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide

Nathan W. Luedtke, Judy S. Hwang, Eileen Nava, Dalia Gut, Moshe Kol, Yitzhak Tor

Research output: Contribution to journalArticlepeer-review

Abstract

Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.

Original languageEnglish
Pages (from-to)5732-5740
Number of pages9
JournalNucleic Acids Research
Volume31
Issue number19
DOIs
StatePublished - 1 Oct 2003

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