TY - JOUR
T1 - The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide
AU - Luedtke, Nathan W.
AU - Hwang, Judy S.
AU - Nava, Eileen
AU - Gut, Dalia
AU - Kol, Moshe
AU - Tor, Yitzhak
N1 - Funding Information:
Financial support was provided, in part, by the National Institutes of Health (AI 47673 and GM 58447 to Y.T.). D.G. and M.K. are grateful to the Israel Science Foundation and the Israel Academy of Sciences and Humanities for support. J.S.H. thanks the Beckman Scholars Program for an undergraduate research fellowship.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.
AB - Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNAPhe, polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Λ- and Δ-[Ru(bpy)2eilatin]2+ have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (Kd ≤ 1 μM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.
UR - http://www.scopus.com/inward/record.url?scp=0141905886&partnerID=8YFLogxK
U2 - 10.1093/nar/gkg758
DO - 10.1093/nar/gkg758
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AN - SCOPUS:0141905886
SN - 0305-1048
VL - 31
SP - 5732
EP - 5740
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 19
ER -
