The determination of intracellular water space by NMR

Hadassah Shinar, Gil Navon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)63- or Co(imidazole)63+ and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalFEBS Letters
Issue number1
StatePublished - 25 Nov 1985


  • Co-NMR
  • Erythrocyte
  • H-NMR
  • Intracellular water space


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