The determination of intracellular water space by NMR

Hadassah Shinar; Gil Navon

doi:10.1016/0014-5793(85)80082-X

The determination of intracellular water space by NMR

Hadassah Shinar, Gil Navon^*

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of ⁵⁹Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)₆^3- or Co(imidazole)₆³⁺ and the ²H or ¹H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.

Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	FEBS Letters
Volume	193
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(85)80082-X
State	Published - 25 Nov 1985

Keywords

Co-NMR
Erythrocyte
H-NMR
Intracellular water space

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abstract = "A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)63- or Co(imidazole)63+ and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.",

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AB - A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)63- or Co(imidazole)63+ and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.

KW - Co-NMR

KW - Erythrocyte

KW - H-NMR

KW - Intracellular water space

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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The determination of intracellular water space by NMR

Abstract

Keywords

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