The determination of intracellular water space by NMR

Hadassah Shinar, Gil Navon

Research output: Contribution to journalArticlepeer-review

Abstract

A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)63- or Co(imidazole)63+ and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalFEBS Letters
Volume193
Issue number1
DOIs
StatePublished - 25 Nov 1985

Keywords

  • Co-NMR
  • Erythrocyte
  • H-NMR
  • Intracellular water space

Fingerprint

Dive into the research topics of 'The determination of intracellular water space by NMR'. Together they form a unique fingerprint.

Cite this