Abstract
A new method for the determination of intracellular water space using NMR spectroscopy is described. The method is based on the measurement of 59Co NMR signal intensity of an inert, stable and membraneimpermeable cobalt(III) compound such as Co(CN)63- or Co(imidazole)63+ and the 2H or 1H NMR signal intensities of the freely permeable water. As an example of the method, the variation of the intracellular water space of human erythrocytes as a function of osmolality was measured.
Original language | English |
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Pages (from-to) | 75-78 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 193 |
Issue number | 1 |
DOIs | |
State | Published - 25 Nov 1985 |
Keywords
- Co-NMR
- Erythrocyte
- H-NMR
- Intracellular water space