The contribution of common rpsL mutations in Escherichia coli to sensitivity to ribosome targeting antibiotics

Gidi Pelchovich, Rachel Schreiber, Alina Zhuravlev, Uri Gophna*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Point mutations in the rpsL gene encoding ribosomal protein S12 can generate resistance to streptomycin, resulting in rapid emergence of resistance to this antibiotic during treatment. In this work, we demonstrate that while spontaneous rpsL mutants in Escherichia coli are resistant to streptomycin, they are more sensitive to the ribosome - targeting antibiotics chloramphenicol, tetracycline and erythromycin. Moreover, combinations of these antibiotics, even in low concentrations were enough to achieve complete growth inhibition of both wild type and rpsL mutant strains. Thus, combining ribosome-targeting drugs can be used as a new treatment strategy that may be effective against streptomycin-resistant ribosome mutants.

Original languageEnglish
Pages (from-to)558-562
Number of pages5
JournalInternational Journal of Medical Microbiology
Volume303
Issue number8
DOIs
StatePublished - Dec 2013

Funding

FundersFunder number
DIP
German-Israeli Project Cooperation

    Keywords

    • Antibiotic resistance
    • Chloramphenicol
    • Erythromycin
    • Gentamicin
    • Ribosomal protein S12
    • RpsL
    • Tetracycline

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