@article{a9e2243fa91143eba8127076f29e421b,
title = "The Collaborative Cross, developing a resource for mammalian systems genetics: A status report of the Wellcome Trust cohort",
abstract = "We report on the progress of a project funded by the Wellcome Trust to produce over 100 recombinant inbred mouse lines as part of the Collaborative Cross (CC) genetic reference panel. These new strains of mice are being derived from a set of eight genetically diverse founders. The genomes of the finished strains will be mosaics of the founder strains' genomes with a high density of independent recombination breakpoints. The CC mice will be available for distribution free of any intellectual property constraints to serve as a community resource for systems genetics studies.",
author = "Iraqi, {Fuad A.} and Gary Churchill and Richard Mott",
note = "Funding Information: Initial funding by the Wellcome Trust in 2005 supported the breeding of upward of 100 lines, initially at the International Livestock Research Institute (ILRI), based in Kenya. The project was transferred to its current location at Tel Aviv University in Israel in 2007. The U.S. Department of Energy (DOE), The Ellison Foundation, and the U.S. National Institutes of Health (NIH) supported another colony of upward of 300 lines in Oak Ridge National Laboratory, Tennessee, USA, with further lines planned. The breeding design ensures that the autosomal genomes of each line will have equal input contributions from each of the founder strains and that recombination events that accumulate during the breeding process are independent between lines. Some care is required to ensure approximate balance of the sex chromosomes, the mitochondrial genome, and their pairwise combinations. An eight-way “funnel” breeding design was used to mix the genomes before inbreeding by brother-sister mating. Labeling the eight founders with the letters A to H, the funnel design first chooses four F matings, say A × B, C × D, E × F, G × H. Then two G matings AB × CD and EF × GH are made, and finally one G mating ABCD × EFGH. All of the Wellcome CC lines were descended from a set of F matings made at The Jackson Laboratory, Bar Harbor, Maine, USA. The F hybrids were distributed to multiple sites where they were mated to produce a balanced set of funnels. The F generations were then sib-mated to produce unique incipient CC recombinant inbred lines (RIL). The inbreeding process is still ongoing. Once inbred, these mice will be available for distribution, free of any intellectual property constraint, and will serve as a powerful tool for quantitative trait analysis and systems biology. 1 2 3 1 1 3 Funding Information: The authors appreciate the generous support of Wellcome Trust for funding this project, and the International Livestock Research Institute (ILRI) and Tel Aviv University for their technical support.",
year = "2008",
month = jun,
doi = "10.1007/s00335-008-9113-1",
language = "אנגלית",
volume = "19",
pages = "379--381",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer",
number = "6",
}