The choice of induction factors for differentiation of multipotent mesenchymal stromal cells derived from human adipose tissue to insulin-producing cells in vitro

Differentiation of mesenchymal stromal cells (MSC) toward the insulin-producing cells is a promising approach for the development of protocol for treatment of patients with type 1 diabetes mellitus. The aim of the study was to assess the capacity of adult human MSC derived from adipose tissue to differentiate in vitro into insulin-producing cells under influence of newborn pig pancreatic extracts and extracts of regenerating rat pancreas in high glucose medium. Adipose tissue MSC had phenotype CD29 ⁺, CD44 ⁺, CD73 ⁺, CD105 ⁺ and CD34 ^-, CD38 ^-, CD45 ^-. During culturing in the medium with high glucose (25 mM) in the presence 10 mM nicotinamide MSC changed their morphology, stained by dithizone, formed clusters and expressed insulin and proinsulin. The presence of pancreatic extracts in culture medium stimulated differentiation of MSC into insulinproducing cells. According to morphological examination, immunohistological and morphometrical analysis of insulin and proinsulin expression the piglet pancreatic extract had the most inducing effect on differentiation MSC forward insulinproducing cells.