The catabolism of lung surfactant by alveolar macrophages

Nora Stern, Shoshana Riklis, Moshe Kalina, Alisa Tietz

Research output: Contribution to journalArticlepeer-review

Abstract

Surfactant was isolated from lung tissue of normal and chlorocyclizine-fed rats. Chlorocyclizine surfactant contained 2.5-3.4 times more phospholipids per mg protein than normal surfactant. Alveolar macrophages, incubated in vitro with normal and Chlorocyclizine surfactants hydrolyzed the surfactant phospholipids and incorporated the fatty acids into cellular triacylglycerol. Employing [3H]palmitate-labeled surfactant, it was shown that cells incubated with Chlorocyclizine surfactant incorporated 46.2-73.0 nmol of fatty acids per mg protein and were transformed into foam cells. Employing fluorescein or 125I-labeled surfactant, the uptake of surfactant protein by macrophages was shown. No significant differences between protein uptake from normal and Chlorocyclizine surfactants were observed. These results suggest that the surfactant phospholipids and protein were catabolized independently.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume877
Issue number3
DOIs
StatePublished - 18 Jul 1986

Keywords

  • (Alveolar macrophage)
  • Chlorocyclizine
  • Lung surfactant
  • Phospholipid breakdown

Fingerprint

Dive into the research topics of 'The catabolism of lung surfactant by alveolar macrophages'. Together they form a unique fingerprint.

Cite this