The biosynthesis of thiamine. IV. Inhibition by vitamin B6 compounds

Lawrence M. Lewin, Gene M. Brown

Research output: Contribution to journalArticlepeer-review


A novel method for the synthesis of the mono- and pyrophosphate esters of 2-methyl-4-amino-5-hydroxymethylpyrimidine has been described. The inhibitory effect of vitamin B6 compounds on the biosynthesis of thiamine has been shown to be due primarily to the inhibition by pyridoxal phosphate of the enzymic reaction whereby thiamine monophosphate is formed from the monophosphate ester of 4-methyl-5-(2-hydroxyethyl)thiazole and the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine. Kinetic experiments indicated that the inhibition is of a "mixed" type, i.e., a mixture of a purely noncompetitive type and a partially competitive type (partially competitive with the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine).

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalArchives of Biochemistry and Biophysics
Issue number2
StatePublished - May 1963


FundersFunder number
National Science Foundation
National Institutes of HealthG-4580


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