TY - JOUR
T1 - The biosynthesis of thiamine conversion of 2-methyl-4-amino-5-formylpyrimidine to 2-methyl-4-amino-5-hydroyxmethylpyrimidine by cell-free extracts of baker's yeast
AU - Wei, R.
AU - Lewin, Lawrence
N1 - Funding Information:
This researchw as partially supportedb y U. S. Public Health ServiceG rant AMo8393a nd by a grant from the WashingtonH eartAssociation.
PY - 1970/2/24
Y1 - 1970/2/24
N2 - 1. 1. Cell-free preparations of Saccharomyces cerevisiae, were shown to catalyze the reduction of 2-methyl-4-amino-5-formylpyrimidine to the corresponding 5-hydroxymethyl derivative, a known intermediate in thiamine biosynthesis. Either NADH or NADPH served as a cofactor. The pH optimum of the system was 6.5-7.0 and the apparent Km for the formylpyrimidine substrate was 3.3·10-4 M. 2. 2. Details of a microbiological assay for the pyrimidine moiety of thiamine, using Escherichia coli Strain M70-17, are described.
AB - 1. 1. Cell-free preparations of Saccharomyces cerevisiae, were shown to catalyze the reduction of 2-methyl-4-amino-5-formylpyrimidine to the corresponding 5-hydroxymethyl derivative, a known intermediate in thiamine biosynthesis. Either NADH or NADPH served as a cofactor. The pH optimum of the system was 6.5-7.0 and the apparent Km for the formylpyrimidine substrate was 3.3·10-4 M. 2. 2. Details of a microbiological assay for the pyrimidine moiety of thiamine, using Escherichia coli Strain M70-17, are described.
UR - http://www.scopus.com/inward/record.url?scp=0014960281&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(70)90308-9
DO - 10.1016/0304-4165(70)90308-9
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AN - SCOPUS:0014960281
SN - 0304-4165
VL - 201
SP - 334
EP - 339
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 2
ER -