The biochemistry of an acetylcholine receptor

M. A. Raftery, R. Vandlen, D. Michaelson, J. Bode, T. Moody, Y. Chao, K. Reed, J. Deutsch, J. Duguid

Research output: Contribution to journalArticlepeer-review


The acetylcholine receptor from Torpedo californica electroplax was studied at 3 levels of molecular organization: receptor rich membrane fragments, solubilized and purified receptor, and reconstituted receptor in phospholipid vesicles. The binding of cholinergic ligands to the membrane bound and the solubilized material is not cooperative, and the number of ligand sites is less than the number of toxin sites. In addition, the purified macromolecule contains the molecular features necessary for ion translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified receptor and Torpedo phospholipids.

Original languageEnglish
Pages (from-to)582-592
Number of pages11
JournalUnknown Journal
Issue number5-6
StatePublished - 1974
Externally publishedYes


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