TY - JOUR
T1 - The biochemistry of an acetylcholine receptor
AU - Raftery, M. A.
AU - Vandlen, R.
AU - Michaelson, D.
AU - Bode, J.
AU - Moody, T.
AU - Chao, Y.
AU - Reed, K.
AU - Deutsch, J.
AU - Duguid, J.
N1 - Funding Information:
We would like to thank the members and staff of the Department of Rheumatology and Immunology of the Second Affiliated Hospital of Soochow University who contributed to this manuscript.
PY - 1974
Y1 - 1974
N2 - The acetylcholine receptor from Torpedo californica electroplax was studied at 3 levels of molecular organization: receptor rich membrane fragments, solubilized and purified receptor, and reconstituted receptor in phospholipid vesicles. The binding of cholinergic ligands to the membrane bound and the solubilized material is not cooperative, and the number of ligand sites is less than the number of toxin sites. In addition, the purified macromolecule contains the molecular features necessary for ion translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified receptor and Torpedo phospholipids.
AB - The acetylcholine receptor from Torpedo californica electroplax was studied at 3 levels of molecular organization: receptor rich membrane fragments, solubilized and purified receptor, and reconstituted receptor in phospholipid vesicles. The binding of cholinergic ligands to the membrane bound and the solubilized material is not cooperative, and the number of ligand sites is less than the number of toxin sites. In addition, the purified macromolecule contains the molecular features necessary for ion translocation during postsynaptic depolarization, since a chemically excitable membrane can be formed from purified receptor and Torpedo phospholipids.
UR - http://www.scopus.com/inward/record.url?scp=0016332514&partnerID=8YFLogxK
U2 - 10.1002/jss.400020506
DO - 10.1002/jss.400020506
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AN - SCOPUS:0016332514
VL - 2
SP - 582
EP - 592
JO - Unknown Journal
JF - Unknown Journal
IS - 5-6
ER -