The Aspergillus nidulans pkcA gene is involved in polarized growth, morphogenesis and maintenance of cell wall integrity

Revital Ronen; Haim Sharon; Emma Levdansky; Jacob Romano; Yona Shadkchan; Nir Osherov

doi:10.1007/s00294-007-0129-y

The Aspergillus nidulans pkcA gene is involved in polarized growth, morphogenesis and maintenance of cell wall integrity

Revital Ronen, Haim Sharon, Emma Levdansky, Jacob Romano, Yona Shadkchan, Nir Osherov^*

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

The protein kinase C (PKC) family participates in maintaining integrity and growth of fungal cell walls. However, the precise molecular role of these proteins in the filamentous fungi remains unknown. In this work, pkcA, the gene encoding the PKC homolog in the filamentous fungus Aspergillus nidulans, was cloned and its function analyzed using a conditional alcA-PKC mutant strain. Repression of pkcA expression resulted in increased conidial swelling, decreased rates of hyphal growth, changes in the ultrastructure of the cell wall and increased sensitivity to antifungal agents. These results suggest that the protein encoded by pkcA is involved in key aspects of cell morphogenesis and cell wall integrity.

Original language	English
Pages (from-to)	321-329
Number of pages	9
Journal	Current Genetics
Volume	51
Issue number	5
DOIs	https://doi.org/10.1007/s00294-007-0129-y
State	Published - May 2007

Keywords

Aspergillus nidulans
Fungal cell wall
pkcA

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10.1007/s00294-007-0129-y

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title = "The Aspergillus nidulans pkcA gene is involved in polarized growth, morphogenesis and maintenance of cell wall integrity",

abstract = "The protein kinase C (PKC) family participates in maintaining integrity and growth of fungal cell walls. However, the precise molecular role of these proteins in the filamentous fungi remains unknown. In this work, pkcA, the gene encoding the PKC homolog in the filamentous fungus Aspergillus nidulans, was cloned and its function analyzed using a conditional alcA-PKC mutant strain. Repression of pkcA expression resulted in increased conidial swelling, decreased rates of hyphal growth, changes in the ultrastructure of the cell wall and increased sensitivity to antifungal agents. These results suggest that the protein encoded by pkcA is involved in key aspects of cell morphogenesis and cell wall integrity.",

keywords = "Aspergillus nidulans, Fungal cell wall, pkcA",

author = "Revital Ronen and Haim Sharon and Emma Levdansky and Jacob Romano and Yona Shadkchan and Nir Osherov",

note = "Funding Information: Acknowledgments This work was funded by the Stoltz Foundation and the Israel Academy of Sciences, grant 741/01 to NO.",

year = "2007",

month = may,

doi = "10.1007/s00294-007-0129-y",

language = "אנגלית",

volume = "51",

pages = "321--329",

journal = "Current Genetics",

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TY - JOUR

T1 - The Aspergillus nidulans pkcA gene is involved in polarized growth, morphogenesis and maintenance of cell wall integrity

AU - Ronen, Revital

AU - Sharon, Haim

AU - Levdansky, Emma

AU - Romano, Jacob

AU - Shadkchan, Yona

AU - Osherov, Nir

N1 - Funding Information: Acknowledgments This work was funded by the Stoltz Foundation and the Israel Academy of Sciences, grant 741/01 to NO.

PY - 2007/5

Y1 - 2007/5

N2 - The protein kinase C (PKC) family participates in maintaining integrity and growth of fungal cell walls. However, the precise molecular role of these proteins in the filamentous fungi remains unknown. In this work, pkcA, the gene encoding the PKC homolog in the filamentous fungus Aspergillus nidulans, was cloned and its function analyzed using a conditional alcA-PKC mutant strain. Repression of pkcA expression resulted in increased conidial swelling, decreased rates of hyphal growth, changes in the ultrastructure of the cell wall and increased sensitivity to antifungal agents. These results suggest that the protein encoded by pkcA is involved in key aspects of cell morphogenesis and cell wall integrity.

AB - The protein kinase C (PKC) family participates in maintaining integrity and growth of fungal cell walls. However, the precise molecular role of these proteins in the filamentous fungi remains unknown. In this work, pkcA, the gene encoding the PKC homolog in the filamentous fungus Aspergillus nidulans, was cloned and its function analyzed using a conditional alcA-PKC mutant strain. Repression of pkcA expression resulted in increased conidial swelling, decreased rates of hyphal growth, changes in the ultrastructure of the cell wall and increased sensitivity to antifungal agents. These results suggest that the protein encoded by pkcA is involved in key aspects of cell morphogenesis and cell wall integrity.

KW - Aspergillus nidulans

KW - Fungal cell wall

KW - pkcA

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U2 - 10.1007/s00294-007-0129-y

DO - 10.1007/s00294-007-0129-y

M3 - מאמר

AN - SCOPUS:34247208120

VL - 51

SP - 321

EP - 329

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 5

ER -

The Aspergillus nidulans pkcA gene is involved in polarized growth, morphogenesis and maintenance of cell wall integrity

Abstract

Keywords

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