The antigenic composition of tumours, sera and urines of tumour-bearing mice and the partial purification of two antigens present in increased amounts

I. Witz, G. Hermann, M. Pikovski, J. Gross

Research output: Contribution to journalArticlepeer-review

Abstract

Growth of mammary carcinoma in mice was shown to be associated with increased concentrations of beta 2 I globulin (transferrin), beta 2 III globulin (a haemoglobin binding protein) and beta 3 I globulin (constituent of the complement) in serum. Soluble extracts of tumour contained marked concentrations of the beta 2 I and the beta 2 III globulins. Three proteins with beta-gamma mobilities were detected and in addition esterase activity was demonstrable in the beta 2 region of the electropherogram. Neither the 3 latter proteins nor the esterase were demonstrable in serum. The transferrin and the haemoglobin binding beta 2 III globulin were partially purified. Normal mouse urine contained at least 5 to 7 proteins of which only two albumin and an alpha globulin were identical with the corresponding serum proteins. Urine from tumour-bearing mice showed additional proteins, immunologically identical with those of serum and tumour extracts. We are obliged to Professor P. Grabar and to Dr. C. de Vaux St. Cyr and Dr. P. Burtin for their constant interest, help, suggestions, and discussions of the work. Part of the studies reported were carried out in the Institut de Recherches Scientifiques sur le Cancer, Villejuif, Seine, France, during the tenure of a stipend from the Anne Frank Foundation (Frankfurt/Main) by I. W., who is particularly grateful for the helpfulness of Professor P. Grabar, Director of the Institut de Recherches Scientifiques sur le Cancer, during his stay there. This report is part of the Ph.D. work of I. W., holder of the Jacob I. Schaffer Scholarship. The careful technical assistance of Miss I. Erk and Miss C. Brunschwig is acknowledged.

Original languageEnglish
Pages (from-to)397-406
Number of pages10
JournalBritish Journal of Cancer
Volume18
Issue number2
DOIs
StatePublished - Jun 1964
Externally publishedYes

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