The anti-DNA-associated idiotype 8.12 IS encoded by the VλII gene family and maps to the vicinity of L chain CDR

Elahna Paul, Andrey A. Iliev, Avi Livneh, Betty Diamond*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The 8.12 idiotype is an anti-DNA-associated Id present on λ L chains that are expressed at high titers in 50% of patients with systemic lupus erythematosus. Since this Id can be present on as much as a third of a patient's anti-DNA antibodies and is found in renal glomeruli, 8.12 is thought to be a marker for a subset of pathogenic anti-DNA autoantibodies. A molecular analysis of the 8.12 positive antibodies was designed to explore the genetic basis of this Id. Monoclonal human B cell lines were generated by transformation with EBV and λ L chainsecreting lines were analyzed for Id expression and V region gene usage. In this panel of IgX cell lines, the 8.12 idiotype is encoded exclusively by members of the VλII gene family. The sequences of several 8.12+ and 8.12- VλII genes are reported here and are used to map the 8.12 Id to the vicinity of CDR1, as well as to further characterize the large and polymorphic VλII gene family.

Original languageEnglish
Pages (from-to)3588-3595
Number of pages8
JournalJournal of Immunology
Volume149
Issue number11
StatePublished - 1992
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteP30CA013330
National Institute of Allergy and Infectious DiseasesP01AI010702
National Institute of Arthritis and Musculoskeletal and Skin DiseasesR37AR032371

    Fingerprint

    Dive into the research topics of 'The anti-DNA-associated idiotype 8.12 IS encoded by the VλII gene family and maps to the vicinity of L chain CDR'. Together they form a unique fingerprint.

    Cite this