TY - JOUR
T1 - The 60-kDa heat shock protein (HSP60) of the sea anemone Anemonia viridis
T2 - A potential early warning system for environmental changes
AU - Choresh, Omer
AU - Ron, Eliora
AU - Loya, Yossi
PY - 2001
Y1 - 2001
N2 - Expression of heat shock proteins (HSPs) is often correlated with adaptation to environmental stress. We examined the role of HSP60 (60 kDa) in acclimatization to thermal stress in the sea anemone Anemonia viridis. Using monoclonal antibodies, we identified HSP60 in sea anemones for the first time, and showed that its expression varied with changes in seawater temperature (SWT). Anemonia viridis displayed high levels of HSP60 when extreme temperatures prevailed in stressful habitats such as tidal pools. Specimens sampled from different temperature layers in the same tidal pool differed in their levels of HSP60. Specimens from subtidal zones exhibited a seasonal pattern of expression of HSP60, according to the seasonal SWT. The level of HSP60 was significantly higher in the summer (SWT, 31°C) than in other seasons throughout the year. This study suggests the use of HSP60 expression as a tool for stress detection in marine invertebrates.
AB - Expression of heat shock proteins (HSPs) is often correlated with adaptation to environmental stress. We examined the role of HSP60 (60 kDa) in acclimatization to thermal stress in the sea anemone Anemonia viridis. Using monoclonal antibodies, we identified HSP60 in sea anemones for the first time, and showed that its expression varied with changes in seawater temperature (SWT). Anemonia viridis displayed high levels of HSP60 when extreme temperatures prevailed in stressful habitats such as tidal pools. Specimens sampled from different temperature layers in the same tidal pool differed in their levels of HSP60. Specimens from subtidal zones exhibited a seasonal pattern of expression of HSP60, according to the seasonal SWT. The level of HSP60 was significantly higher in the summer (SWT, 31°C) than in other seasons throughout the year. This study suggests the use of HSP60 expression as a tool for stress detection in marine invertebrates.
KW - Acclimatization
KW - Anemonia viridis
KW - Early warning system
KW - Environmental stress
KW - HSP60
KW - Heat shock proteins
KW - Immunoblotting
KW - Mediterranean
UR - http://www.scopus.com/inward/record.url?scp=0035667793&partnerID=8YFLogxK
U2 - 10.1007/s10126-001-0007-4
DO - 10.1007/s10126-001-0007-4
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AN - SCOPUS:0035667793
SN - 1436-2228
VL - 3
SP - 501
EP - 508
JO - Marine Biotechnology
JF - Marine Biotechnology
IS - 5
ER -