TY - JOUR
T1 - Thawing day 3 embryos and culturing to day 5 may be a better method for frozen embryo transfer
AU - Rahav-Koren, Roni
AU - Inbar, Shmuel
AU - Miller, Netanella
AU - Wiser, Amir
AU - Yagur, Yael
AU - Berkowitz, Chen
AU - Farladansky-Gershnabel, Sivan
AU - Shulman, Adrian
AU - Berkowitz, Arie
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/11
Y1 - 2021/11
N2 - Purpose: Does thawing cleavage embryos and culturing them for transfer as blastocysts improve pregnancy and perinatal outcomes compared to transferring thawed blastocysts? Methods: Retrospective, observational cohort study performed at two assisted reproductive technology centers, 2014 to 2020. A total of 450 patients with 463 thawed embryo transfer cycles were divided into 2 groups according to the embryonic developmental stage at cryopreservation and transfer: 231 thawed blastocysts (day 5 group) and 232 thawed cleavage embryos that were cultured for 2 days and transferred as blastocysts (day 3–5 group). The two groups were compared for demographics, routine parameters of IVF treatment, pregnancy rates, and perinatal outcomes. Results: Multivariable logistic regression analysis for ongoing pregnancy and delivery demonstrated that the day 3–5 group had a greater likelihood of achieving ongoing pregnancy and delivery compared to the day 5 group (OR 1.58, 95%CI 1.062–2.361, p = 0.024). Perinatal outcomes were comparable between the three groups. Conclusion: Our results support culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts to increase chances of ongoing pregnancy and delivery.
AB - Purpose: Does thawing cleavage embryos and culturing them for transfer as blastocysts improve pregnancy and perinatal outcomes compared to transferring thawed blastocysts? Methods: Retrospective, observational cohort study performed at two assisted reproductive technology centers, 2014 to 2020. A total of 450 patients with 463 thawed embryo transfer cycles were divided into 2 groups according to the embryonic developmental stage at cryopreservation and transfer: 231 thawed blastocysts (day 5 group) and 232 thawed cleavage embryos that were cultured for 2 days and transferred as blastocysts (day 3–5 group). The two groups were compared for demographics, routine parameters of IVF treatment, pregnancy rates, and perinatal outcomes. Results: Multivariable logistic regression analysis for ongoing pregnancy and delivery demonstrated that the day 3–5 group had a greater likelihood of achieving ongoing pregnancy and delivery compared to the day 5 group (OR 1.58, 95%CI 1.062–2.361, p = 0.024). Perinatal outcomes were comparable between the three groups. Conclusion: Our results support culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts to increase chances of ongoing pregnancy and delivery.
KW - Blastocyst
KW - Cleavage
KW - Thawed embryo transfer
KW - Vitrification
UR - http://www.scopus.com/inward/record.url?scp=85115225457&partnerID=8YFLogxK
U2 - 10.1007/s10815-021-02321-y
DO - 10.1007/s10815-021-02321-y
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C2 - 34549329
AN - SCOPUS:85115225457
SN - 1058-0468
VL - 38
SP - 2941
EP - 2946
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 11
ER -