TY - JOUR
T1 - (TG)n uncovers a sex-specific hybridization pattern in cattle
AU - Kashi, Yechezkel
AU - Iraqi, Fuad
AU - Tikochinski, Yaron
AU - Ruzitsky, Bracha
AU - Nave, Alona
AU - Beckmann, Jacques S.
AU - Friedmann, Adam
AU - Soller, Morris
AU - Gruenbaum, Yosef
N1 - Funding Information:
We thank Dr. A. Jeffreys for providing the 33.6 and 33.15 probes, and the Hasherut and Ha-On Artificial Insemination Centers for the bovine blood samples. The research by Y.K. and F.I. was supported by the Israel Ministry of Research and Development and the National Council For Research and Development. This research was supported by the US-Israel Binational Agricultural Research and Development Fund (BARD).
PY - 1990/5
Y1 - 1990/5
N2 - Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.
AB - Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.
UR - http://www.scopus.com/inward/record.url?scp=0025195159&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(90)90515-V
DO - 10.1016/0888-7543(90)90515-V
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AN - SCOPUS:0025195159
SN - 0888-7543
VL - 7
SP - 31
EP - 36
JO - Genomics
JF - Genomics
IS - 1
ER -