Tetracycline impregnation affects degradation of porcine collagen matrix in healthy and diabetic rats

Haim Tal*, Miron Weinreb, Asaf Shely, Carlos E. Nemcovsky, Ofer Moses

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objectives: The present study evaluated the degradation of collagen matrix (CM) immersed in tetracycline (TTC) or phosphate-buffered saline (PBS) in diabetic and normoglycemic rats. Materials and methods: Diabetes was induced in 15 rats by systemic streptozotocin (STZ) (experimental); 15 healthy rats served as controls. One day before implantation 60 CM disks, 5 mm in diameter, were labeled with biotin: 30 were immersed in tetracycline (TTC) and 30 in PBS. One disk of each type was implanted subdermally in each rat. Animals were euthanized after 3 weeks, and tissue specimens containing the disks were prepared for histologic analysis. Horseradish peroxidase (HRP)-conjugated streptavidin was used to detect the remaining biotinylated collagen. Residual collagen area within the CM disks was analyzed and compared to baseline. Results: Diabetes significantly increased the CM degradation. Immersion of the CM disks in a 50-mg/mL TTC solution before implantation decreased its degradation both in diabetic and normoglycemic rats. Conclusions: Diabetes significantly increases collagen matrix degradation; immersion of collagen matrix in TTC before implantation decreases its degradation in both diabetic and normoglycemic conditions. Clinical relevance: Immersion of medical collagen devices in TTC may be an effective means to decrease their resorption rate and increase their effectiveness, especially in situations with increased degradation such as diabetes.

Original languageEnglish
Pages (from-to)1237-1242
Number of pages6
JournalClinical Oral Investigations
Issue number6
StatePublished - 1 Jul 2016


  • Animal model
  • Biodegradation
  • Collagen
  • Diabetes
  • Membranes


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