Testosterone stimulates growth of tibial epiphyseal growth plate and insulin-like growth factor-1 receptor abundance in hypophysectomized and castrated rats

M. Phillip, G. Maor, S. Assa, A. Silbergeld, Y. Segev

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Puberty is associated with an increase in the plasma concentration of sex steroids, growth hormone (GH), and insulin-like growth factor-1 (IGF-1). Gonadal steroid hormones are important for the normal pubertal growth spurt and skeletal growth. The mechanism by which gonadal steroids induces skeletal growth is still not fully understood. To study the GH-independent effect of testosterone on growth, we investigated the effect of testosterone injections on the tibial epiphyseal growth plate (EGP) in an in vivo model of hypophysectomized and castrated male rats. Four groups (six animais each) of 28-d-old male rats were studied. Groups A, B, and C were hypophysectomized and castrated and received 500 μg/(kg·d) of hydrocortisone and 15 μg/(kg·d) of levothyroxine sodium. Groups A and B were also treated with daily sc injections of 10 μg of testosterone/100 g of body wt and 100 μg of testosterone/100 g of body wt, respectively, for 7 d. Group C was injected with vehicle alone. Group D were intact animais injected with saline (controls). Animais were sacrificed on 8 d. As expected, serum GH levels were found to be very low (1.13 ± 0.1 ng/ mL) in the hypophysectomized animais (group C, hypopit), and testosterone treatment did not change them significantly. Serum IGF-1 decreased from 502.9 ± 13 ng/mL in group D to 167 ± 41.4 ng/mL in group C (p < 0.001). Testosterone therapy had no stimulatory effect on serum IGF-1 levels in the hypopit + low-dose group (A) (220 ± 94.8 ng/mL) and had an inhibitory effect in the hypopit + high-dose group (B) (39.3 ± 17.5). Histomorphometric determinations demonstrated an EGP width of 472.3 ± 39 μm in the intact animais but only 336.9 ± 1.6 μm in the hypopit group (C) (p < 0.01). High-dose testosterone treatment (group B) significantly increased the EGP width (to 438.8 ± 27.8), (p < 0.001), whereas low-dose testosterone (group A) did not. Immunohistochemistry studies revealed that the levels of IGF-1 in the EGP of the control animais were almost negligible and that testosterone did not change them. However, testosterone increased in a dose-dependent manner the abundance of IGF-1 receptor EGP. We conclude that testosterone has a direct, local, GH-independent effect on the EGP growth and IGF-1 receptor abundance.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
Issue number1
StatePublished - 2001
Externally publishedYes


  • Epiphyseal growth plate
  • IGF-1
  • Testosterone


Dive into the research topics of 'Testosterone stimulates growth of tibial epiphyseal growth plate and insulin-like growth factor-1 receptor abundance in hypophysectomized and castrated rats'. Together they form a unique fingerprint.

Cite this