Tentoxin sensitivity of chloroplasts determined by Codon 83 of β subunit of proton-ATPase

Adi Avni*, James D. Anderson, Neta Holland, Jean David Rochaix, Zippora Gromet-Elhanan, Marvin Edelman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Tentoxin is a naturally occurring phytotoxic peptide that causes seedling chlorosis and arrests growth in sensitive plants and algae. In vitro, it inhibits activity of the β subunit of the plastid proton-adenosine triphosphatase (ATPase) from sensitive species. Plastid atpB genes from six closely related, tentoxin-sensitive or -resistant Nicotiana species differ at codon 83, according to their response to the toxin: glutamate correlated with resistance and aspartate correlated with sensitivity. The genetic relevance of this site was confirmed in Chlamydomonas reinhardtii by chloroplast transformation. The alga, normally tentoxin-resistant, was rendered tentoxin-sensitive by mutagenesis of its plastid atpB gene at codon 83. Codon 83 may represent a critical site on the β subunit that does not compete with nucleotide binding or other catalytic activities.

Original languageEnglish
Pages (from-to)1245-1247
Number of pages3
JournalScience
Volume257
Issue number5074
DOIs
StatePublished - 1992
Externally publishedYes

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