Templating S100A9 amyloids on Aβ fibrillar surfaces revealed by charge detection mass spectrometry, microscopy, kinetic and microfluidic analyses

Jonathan Pansieri, Igor A. Iashchishyn, Hussein Fakhouri, Lucija Ostojić, Mantas Malisauskas, Greta Musteikyte, Vytautas Smirnovas, Matthias M. Schneider, Tom Scheidt, Catherine K. Xu, Georg Meisl, Tuomas P.J. Knowles, Ehud Gazit, Rodolphe Antoine, Ludmilla A. Morozova-Roche

Research output: Contribution to journalArticlepeer-review

Abstract

The mechanism of amyloid co-aggregation and its nucleation process are not fully understood in spite of extensive studies. Deciphering the interactions between proinflammatory S100A9 protein and Aβ42 peptide in Alzheimer's disease is fundamental since inflammation plays a central role in the disease onset. Here we use innovative charge detection mass spectrometry (CDMS) together with biophysical techniques to provide mechanistic insight into the co-aggregation process and differentiate amyloid complexes at a single particle level. Combination of mass and charge distributions of amyloids together with reconstruction of the differences between them and detailed microscopy reveals that co-aggregation involves templating of S100A9 fibrils on the surface of Aβ42 amyloids. Kinetic analysis further corroborates that the surfaces available for the Aβ42 secondary nucleation are diminished due to the coating by S100A9 amyloids, while the binding of S100A9 to Aβ42 fibrils is validated by a microfluidic assay. We demonstrate that synergy between CDMS, microscopy, kinetic and microfluidic analyses opens new directions in interdisciplinary research. This journal is

Original languageEnglish
Article numberc9sc05905a
Pages (from-to)7031-7039
Number of pages9
JournalChemical Science
Volume11
Issue number27
DOIs
StatePublished - 21 Jul 2020

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