Tc99m macroaggregated albumin cisternography for the detection of persistent cerebrospinal fluid (CSF) leak in an animal model

Alexander Zeidel, Moshe M. Melloul, Eduard Maiburd, Dov Dicker, Benzion Beilin

Research output: Contribution to journalArticlepeer-review

Abstract

The present study describes a new in vivo animal model that enables the detection of cerebrospinal fluid (CSF) leakage after dural injury. A polyethylene catheter (PE 10) was inserted into the subarachnoid space in the lumbar area by a simple surgical procedure and a radioactive isotope Tc99m Macroaggregated Albumin (Tc99m MAA) was injected into the CSF. In the experimental group, a standardised dural puncture was performed in the cervical area. The accumulation of the isotope in the gauze placed over the dural puncture and viewed by a gamma camera as a spot of concentrated radioactivity, was indicative of CSF leakage. In a second group of animals with intact cervical dura the absence of leakage was presented as a picture of sporadic background radioactivity. To demonstrate the effectiveness of the model in detection of invisible leakage, blood was applied over the cervical dural defect in another group of animals and CSF leakage was assessed by the above mentioned isotope detection method. This in vivo model may be used for evaluation of the sealing properties of various materials under physiological and metabolic processes in living tissue.

Original languageEnglish
Pages (from-to)181-183
Number of pages3
JournalJournal of Neuroscience Methods
Volume114
Issue number2
DOIs
StatePublished - 15 Mar 2002

Keywords

  • Cerebrospinal fluid leakage
  • Dural puncture
  • Radioactive isotope Tc MAA
  • Radionuclide cisternography

Fingerprint

Dive into the research topics of 'Tc99m macroaggregated albumin cisternography for the detection of persistent cerebrospinal fluid (CSF) leak in an animal model'. Together they form a unique fingerprint.

Cite this