TY - JOUR
T1 - Targeting Persistent Biofilm Infections
T2 - Reconsidering the Topography of the Infection Site during Model Selection
AU - Kolodkin-Gal, Ilana
AU - Cohen-Cymberknoh, Malena
AU - Zamir, Gideon
AU - Tsesis, Igor
AU - Rosen, Eyal
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6
Y1 - 2022/6
N2 - The physiology of an organism in the environment reflects its interactions with the diverse physical, chemical, and biological properties of the surface. These principles come into consideration during model selection to study biofilm–host interactions. Biofilms are communities formed by beneficial and pathogenic bacteria, where cells are held together by a structured extracellular matrix. When biofilms are associated with a host, chemical gradients and their origins become highly relevant. Conventional biofilm laboratory models such as multiwall biofilm models and agar plate models poorly mimic these gradients. In contrast, ex vivo models possess the partial capacity to mimic the conditions of tissue-associated biofilm and a biofilm associated with a mineralized surface enriched in inorganic components, such as the human dentin. This review will highlight the progress achieved using these settings for two models of persistent infections: the infection of the lung tissue by Pseudomonas aeruginosa and the infection of the root canal by Enterococcus faecalis. For both models, we conclude that the limitations of the conventional in vitro systems necessitate a complimentary experimentation with clinically relevant ex vivo models during therapeutics development.
AB - The physiology of an organism in the environment reflects its interactions with the diverse physical, chemical, and biological properties of the surface. These principles come into consideration during model selection to study biofilm–host interactions. Biofilms are communities formed by beneficial and pathogenic bacteria, where cells are held together by a structured extracellular matrix. When biofilms are associated with a host, chemical gradients and their origins become highly relevant. Conventional biofilm laboratory models such as multiwall biofilm models and agar plate models poorly mimic these gradients. In contrast, ex vivo models possess the partial capacity to mimic the conditions of tissue-associated biofilm and a biofilm associated with a mineralized surface enriched in inorganic components, such as the human dentin. This review will highlight the progress achieved using these settings for two models of persistent infections: the infection of the lung tissue by Pseudomonas aeruginosa and the infection of the root canal by Enterococcus faecalis. For both models, we conclude that the limitations of the conventional in vitro systems necessitate a complimentary experimentation with clinically relevant ex vivo models during therapeutics development.
KW - Enterococcus faecalis
KW - Pseudomonas aeruginosa
KW - biofilm
KW - dentin
KW - experimental models
KW - infection
UR - http://www.scopus.com/inward/record.url?scp=85131299649&partnerID=8YFLogxK
U2 - 10.3390/microorganisms10061164
DO - 10.3390/microorganisms10061164
M3 - מאמר
C2 - 35744683
AN - SCOPUS:85131299649
VL - 10
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 6
M1 - 1164
ER -