Tandem Domains with Tuned Interactions Are a Powerful Biological Design Principle

Ruth Nussinov*, Chung Jung Tsai

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Allosteric effects of mutations, ligand binding, or post-translational modifications on protein function occur through changes to the protein’s shape, or conformation. In a cell, there are many copies of the same protein, all experiencing these perturbations in a dynamic fashion and fluctuating through different conformations and activity states. According to the “conformational selection and population shift” theory, ligand binding selects a particular conformation. This perturbs the ensemble and induces a population shift. In a new PLOS Biology paper, Melacini and colleagues describe a novel model of protein regulation, the “Double-Conformational Selection Model”, which demonstrates how two tandem ligand-binding domains interact to regulate protein function. Here we explain how tandem domains with tuned interactions—but not single domains—can provide a blueprint for sensitive activation sensors within a narrow window of ligand concentration, thereby promoting signaling control.

Original languageEnglish
Article numbere1002306
JournalPLoS Biology
Issue number11
StatePublished - 30 Nov 2015


FundersFunder number
National Cancer Institute
National Institutes of HealthHHSN261200800001E


    Dive into the research topics of 'Tandem Domains with Tuned Interactions Are a Powerful Biological Design Principle'. Together they form a unique fingerprint.

    Cite this