TY - JOUR
T1 - T cell control of staphylococcal enterotoxin B (SEB) lethal sensitivity in mice
T2 - CD4+CD45RB(bright)/CD4+CD45RB(dim) balance defines susceptibility to SEB toxicity
AU - Canaan, Allon
AU - Marcus, Hadar
AU - Burakova, Tanya
AU - David, Madga
AU - Dekel, Benjamin
AU - Segall, Harry
AU - Reisner, Yair
PY - 1999
Y1 - 1999
N2 - Radiation chimeras, generated by transplantation of SCID bone marrow into C3H/HeJ mice, show lethal susceptibility to staphylococcal enterotoxin B (SEB), thus constituting a valid murine model for SEB shock. This SEB sensitivity is due to the ability of the irradiated host to restore residual T cell populations, since the SCID donor bone marrow is unable to generate T cells. SCID bone marrow transplanted into irradiated nude mice does not generate SEB-sensitive chimeras, as a consequence of the inability of the recipient nude mice to develop mature T cells. Thymectomy of normal recipient mice prior to bone marrow transplantation does not affect the development of susceptibility to SEB, suggesting that post-thymic, residual T cells of the host probably mediate this SEB sensitivity. In vivo depletion experiments show that CD4+ T cells are required for the SEB-triggered shock, while CD8+ cells suppress it. A further examination of the T helper subpopulations in the SEB-sensitive mice reveals a prevalence of CD4+CD45RB(dim) cells over CD4+CD45RB(bright) cells. This T helper balance was statistically significant when correlated with SEB-induced mortality. Our model provides a possible explanation for the SEB resistance of normal mice: they have a prevalence of CD4+CD45RB(dim) over CD4+ CD45RB(bright) cells.
AB - Radiation chimeras, generated by transplantation of SCID bone marrow into C3H/HeJ mice, show lethal susceptibility to staphylococcal enterotoxin B (SEB), thus constituting a valid murine model for SEB shock. This SEB sensitivity is due to the ability of the irradiated host to restore residual T cell populations, since the SCID donor bone marrow is unable to generate T cells. SCID bone marrow transplanted into irradiated nude mice does not generate SEB-sensitive chimeras, as a consequence of the inability of the recipient nude mice to develop mature T cells. Thymectomy of normal recipient mice prior to bone marrow transplantation does not affect the development of susceptibility to SEB, suggesting that post-thymic, residual T cells of the host probably mediate this SEB sensitivity. In vivo depletion experiments show that CD4+ T cells are required for the SEB-triggered shock, while CD8+ cells suppress it. A further examination of the T helper subpopulations in the SEB-sensitive mice reveals a prevalence of CD4+CD45RB(dim) cells over CD4+CD45RB(bright) cells. This T helper balance was statistically significant when correlated with SEB-induced mortality. Our model provides a possible explanation for the SEB resistance of normal mice: they have a prevalence of CD4+CD45RB(dim) over CD4+ CD45RB(bright) cells.
KW - CD45RB
KW - Staphylococcal enterotoxin B
KW - Superantigen
KW - Th1
KW - Th2
UR - http://www.scopus.com/inward/record.url?scp=0032933447&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-4141(199904)29:04<1375::AID-IMMU1375>3.0.CO;2-N
DO - 10.1002/(SICI)1521-4141(199904)29:04<1375::AID-IMMU1375>3.0.CO;2-N
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C2 - 10229105
AN - SCOPUS:0032933447
SN - 0014-2980
VL - 29
SP - 1375
EP - 1382
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -