Systematic investigation of cytokine signaling activity at the tissue and single-cell levels

Peng Jiang, Yu Zhang, Beibei Ru, Yuan Yang, Trang Vu, Rohit Paul, Amer Mirza, Grégoire Altan-Bonnet, Lingrui Liu, Eytan Ruppin, Lalage Wakefield, Kai W. Wucherpfennig

Research output: Contribution to journalArticlepeer-review


Cytokines are critical for intercellular communication in human health and disease, but the investigation of cytokine signaling activity has remained challenging due to the short half-lives of cytokines and the complexity/redundancy of cytokine functions. To address these challenges, we developed the Cytokine Signaling Analyzer (CytoSig;, providing both a database of target genes modulated by cytokines and a predictive model of cytokine signaling cascades from transcriptomic profiles. We collected 20,591 transcriptome profiles for human cytokine, chemokine and growth factor responses. This atlas of transcriptional patterns induced by cytokines enabled the reliable prediction of signaling activities in distinct cell populations in infectious diseases, chronic inflammation and cancer using bulk and single-cell transcriptomic data. CytoSig revealed previously unidentified roles of many cytokines, such as BMP6 as an anti-inflammatory factor, and identified candidate therapeutic targets in human inflammatory diseases, such as CXCL8 for severe coronavirus disease 2019.

Original languageEnglish
Pages (from-to)1181-1191
Number of pages11
JournalNature Methods
Issue number10
StatePublished - Oct 2021
Externally publishedYes


Dive into the research topics of 'Systematic investigation of cytokine signaling activity at the tissue and single-cell levels'. Together they form a unique fingerprint.

Cite this