TY - JOUR
T1 - Systematic Identification of Rhythmic Genes Reveals camk1gb as a New Element in the Circadian Clockwork
AU - Tovin, Adi
AU - Alon, Shahar
AU - Ben-Moshe, Zohar
AU - Mracek, Philipp
AU - Vatine, Gad
AU - Foulkes, Nicholas S.
AU - Jacob-Hirsch, Jasmine
AU - Rechavi, Gideon
AU - Toyama, Reiko
AU - Coon, Steven L.
AU - Klein, David C.
AU - Eisenberg, Eli
AU - Gothilf, Yoav
PY - 2012/12
Y1 - 2012/12
N2 - A wide variety of biochemical, physiological, and molecular processes are known to have daily rhythms driven by an endogenous circadian clock. While extensive research has greatly improved our understanding of the molecular mechanisms that constitute the circadian clock, the links between this clock and dependent processes have remained elusive. To address this gap in our knowledge, we have used RNA sequencing (RNA-seq) and DNA microarrays to systematically identify clock-controlled genes in the zebrafish pineal gland. In addition to a comprehensive view of the expression pattern of known clock components within this master clock tissue, this approach has revealed novel potential elements of the circadian timing system. We have implicated one rhythmically expressed gene, camk1gb, in connecting the clock with downstream physiology of the pineal gland. Remarkably, knockdown of camk1gb disrupts locomotor activity in the whole larva, even though it is predominantly expressed within the pineal gland. Therefore, it appears that camk1gb plays a role in linking the pineal master clock with the periphery.
AB - A wide variety of biochemical, physiological, and molecular processes are known to have daily rhythms driven by an endogenous circadian clock. While extensive research has greatly improved our understanding of the molecular mechanisms that constitute the circadian clock, the links between this clock and dependent processes have remained elusive. To address this gap in our knowledge, we have used RNA sequencing (RNA-seq) and DNA microarrays to systematically identify clock-controlled genes in the zebrafish pineal gland. In addition to a comprehensive view of the expression pattern of known clock components within this master clock tissue, this approach has revealed novel potential elements of the circadian timing system. We have implicated one rhythmically expressed gene, camk1gb, in connecting the clock with downstream physiology of the pineal gland. Remarkably, knockdown of camk1gb disrupts locomotor activity in the whole larva, even though it is predominantly expressed within the pineal gland. Therefore, it appears that camk1gb plays a role in linking the pineal master clock with the periphery.
UR - http://www.scopus.com/inward/record.url?scp=84872038509&partnerID=8YFLogxK
U2 - 10.1371/journal.pgen.1003116
DO - 10.1371/journal.pgen.1003116
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:84872038509
SN - 1553-7390
VL - 8
JO - PLoS Genetics
JF - PLoS Genetics
IS - 12
M1 - e1003116
ER -