Systematic antigenic profiling of hematopoietic antigens on ovarian carcinoma cells identifies membrane proteins for targeted therapy development

Limor Helpman, Ben Zion Katz, Tamar Safra, Letizia Schreiber, Zurit Levine, Sergey Nemzer, Yaron Kinar, Dan Grisaru*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: We studied ovarian cancers for the expression of membrane markers of hematopoietic origin. Study Design: We used flow cytometry to systematically characterize the expression of more than 30 hematologic antigens on ovarian carcinoma cell lines and to assess their stability under estrogen exposure. The expression of the antigens was validated by a bioinformatics survey and immunohistochemical staining of ovarian cancer specimens. Results: Several antigens were expressed by the majority of the cells, such as CD15, CD71, and CD138, whereas others were found on small and distinct cellular subpopulations. The expression patterns of the different markers were unaffected by estrogen exposure, indicating their stability. Conclusion: The antigens described in our work may serve as potential targets for new and existing targeted drugs.

Original languageEnglish
Pages (from-to)196.e1-196.e7
JournalAmerican Journal of Obstetrics and Gynecology
Volume201
Issue number2
DOIs
StatePublished - Aug 2009

Keywords

  • bioinformatics
  • flow cytometry
  • hematopoietic antigens
  • ovarian cancer

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