Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain; Chethan D. Shanthamurthy; Shani Leviatan Ben-Arye; Sharon Yehuda; Sharvani S. Nandikol; Hirekodathakallu V. Thulasiram; Vered Padler-Karavani; Raghavendra Kikkeri

doi:10.1039/d1cc00964h

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu V. Thulasiram, Vered Padler-Karavani^*, Raghavendra Kikkeri

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF₁₆₅, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO₃⁻)-IdoA(2-O-SO₃⁻) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF₁₆₅growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF₁₆₅andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

Original language	English
Pages (from-to)	3516-3519
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	28
DOIs	https://doi.org/10.1039/d1cc00964h
State	Published - 11 Apr 2021

Access to Document

10.1039/d1cc00964h

Cite this

@article{bd4606fffa7848fa80255674fdd53c60,

title = "Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis",

abstract = "We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.",

author = "Prashant Jain and Shanthamurthy, {Chethan D.} and {Leviatan Ben-Arye}, Shani and Sharon Yehuda and Nandikol, {Sharvani S.} and Thulasiram, {Hirekodathakallu V.} and Vered Padler-Karavani and Raghavendra Kikkeri",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = apr,

day = "11",

doi = "10.1039/d1cc00964h",

language = "אנגלית",

volume = "57",

pages = "3516--3519",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "28",

}

TY - JOUR

T1 - Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

AU - Jain, Prashant

AU - Shanthamurthy, Chethan D.

AU - Leviatan Ben-Arye, Shani

AU - Yehuda, Sharon

AU - Nandikol, Sharvani S.

AU - Thulasiram, Hirekodathakallu V.

AU - Padler-Karavani, Vered

AU - Kikkeri, Raghavendra

PY - 2021/4/11

Y1 - 2021/4/11

N2 - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

AB - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

UR - http://www.scopus.com/inward/record.url?scp=85103725519&partnerID=8YFLogxK

U2 - 10.1039/d1cc00964h

DO - 10.1039/d1cc00964h

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 33704312

AN - SCOPUS:85103725519

SN - 1359-7345

VL - 57

SP - 3516

EP - 3519

JO - Chemical Communications

JF - Chemical Communications

IS - 28

ER -

Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this