TY - JOUR
T1 - Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis
AU - Jain, Prashant
AU - Shanthamurthy, Chethan D.
AU - Leviatan Ben-Arye, Shani
AU - Yehuda, Sharon
AU - Nandikol, Sharvani S.
AU - Thulasiram, Hirekodathakallu V.
AU - Padler-Karavani, Vered
AU - Kikkeri, Raghavendra
N1 - Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/4/11
Y1 - 2021/4/11
N2 - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.
AB - We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3−)-IdoA(2-O-SO3−) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.
UR - http://www.scopus.com/inward/record.url?scp=85103725519&partnerID=8YFLogxK
U2 - 10.1039/d1cc00964h
DO - 10.1039/d1cc00964h
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C2 - 33704312
AN - SCOPUS:85103725519
SN - 1359-7345
VL - 57
SP - 3516
EP - 3519
JO - Chemical Communications
JF - Chemical Communications
IS - 28
ER -