Synthetic heparan sulfate ligands for vascular endothelial growth factor to modulate angiogenesis

Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu V. Thulasiram, Vered Padler-Karavani*, Raghavendra Kikkeri

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We report the discovery of a potential heparan sulfate (HS) ligand to target several growth factors using 13 unique HS tetrasaccharide ligands. By employing an HS microarray and SPR, we deciphered the crucial structure-binding relationship of these glycans with the growth factors BMP2, VEGF165, HB-EGF, and FGF2. Notably,GlcNHAc(6-O-SO3)-IdoA(2-O-SO3) (HT-2,6S-NAc)tetrasaccharide showed strong binding with the VEGF165growth factor.In vitrovascular endothelial cell proliferation, migration and angiogenesis was inhibited in the presence of VEGF165andHT-2,6S-NAcorHT-6S-NAc, revealing the potential therapeutic role of these synthetic HS ligands.

Original languageEnglish
Pages (from-to)3516-3519
Number of pages4
JournalChemical Communications
Volume57
Issue number28
DOIs
StatePublished - 11 Apr 2021

Funding

FundersFunder number
Department of Biotechnology, Ministry of Science and Technology, IndiaBT/PR21934/NNT/28/1242/ 2017, SERB/F/9228/2019–2020, STARS/APR2019/CS/426/FS
Department of Science and Technology, Ministry of Science and Technology, IndiaSR/ NM/NS-1113/2016
Israel Science Foundation
Indian Institute of Science Education and Research Pune

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