Synthesis of selenium-based BOPHY sensor for imaging of Cu(II) in living HeLa cells

Gauri S. Malankar; Divyesh S. Shelar; M. Manikandan; Malay Patra; Sudesh T. Manjare

doi:10.1016/j.molstruc.2023.135118

Synthesis of selenium-based BOPHY sensor for imaging of Cu(II) in living HeLa cells

Gauri S. Malankar, Divyesh S. Shelar, M. Manikandan, Malay Patra^*, Sudesh T. Manjare

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

NSe₂ chelating moiety embedded BOPHY probe was synthesized and characterized. The probe displayed high selectivity and sensitivity for the detection of Cu(II). The probe reacted with Cu(II) in less than 1 s and showed a 6 fold increment in fluorescence intensity. Detection limit of the probe was found to be 10.8 nM and the binding ratio of the complex (Probe:Cu(II)) was determined to be 1:1 from the Job's plot. In addition, the probe exhibited reversibility with S²⁻ ions. The probe possessed cell-membrane permeability and sensitive detection of Cu(II) in live HeLa cells with strong green fluorescence.

Original language	English
Article number	135118
Journal	Journal of Molecular Structure
Volume	1281
DOIs	https://doi.org/10.1016/j.molstruc.2023.135118
State	Published - 5 Jun 2023
Externally published	Yes

Keywords

BOPHY
Bioimaging
Cu(II) sensor
HeLa cells
Organoselenium
Turn-on fluorescence

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10.1016/j.molstruc.2023.135118

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title = "Synthesis of selenium-based BOPHY sensor for imaging of Cu(II) in living HeLa cells",

abstract = "NSe2 chelating moiety embedded BOPHY probe was synthesized and characterized. The probe displayed high selectivity and sensitivity for the detection of Cu(II). The probe reacted with Cu(II) in less than 1 s and showed a 6 fold increment in fluorescence intensity. Detection limit of the probe was found to be 10.8 nM and the binding ratio of the complex (Probe:Cu(II)) was determined to be 1:1 from the Job's plot. In addition, the probe exhibited reversibility with S2− ions. The probe possessed cell-membrane permeability and sensitive detection of Cu(II) in live HeLa cells with strong green fluorescence.",

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AU - Malankar, Gauri S.

AU - Shelar, Divyesh S.

AU - Manikandan, M.

AU - Patra, Malay

AU - Manjare, Sudesh T.

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AB - NSe2 chelating moiety embedded BOPHY probe was synthesized and characterized. The probe displayed high selectivity and sensitivity for the detection of Cu(II). The probe reacted with Cu(II) in less than 1 s and showed a 6 fold increment in fluorescence intensity. Detection limit of the probe was found to be 10.8 nM and the binding ratio of the complex (Probe:Cu(II)) was determined to be 1:1 from the Job's plot. In addition, the probe exhibited reversibility with S2− ions. The probe possessed cell-membrane permeability and sensitive detection of Cu(II) in live HeLa cells with strong green fluorescence.

KW - BOPHY

KW - Bioimaging

KW - Cu(II) sensor

KW - HeLa cells

KW - Organoselenium

KW - Turn-on fluorescence

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Synthesis of selenium-based BOPHY sensor for imaging of Cu(II) in living HeLa cells

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