@article{61e98350ed1d45bdaaf58c0a72b8d61e,
title = "Synthesis of novel poly(dG)-poly(dG)-poly(dC) triplex structure by Klenow exo- fragment of DNA polymerase I",
abstract = "The extension of the G-strand of long (700 bp) poly(dG)-poly(dC) by the Klenow exo- fragment of DNA polymerase I yields a complete triplex structure of the H-DNA type. High-performance liquid chromatography analysis demonstrates that the length of the G-strand is doubled during the polymerase synthesis. Fluorescence resonance energy transfer analysis shows that the 5′ ends of the G- and the C-strands, labeled with fluorescein and TAMRA, respectively, are positioned close to each other in the product of the synthesis. Atomic force microscopy morphology imaging shows that the synthesized structures lack single-stranded fragments and have approximately the same length as the parent 700 bp poly(dG)-poly(dC). CD spectrum of the polymer has a large negative peak at 278 nm, which is characteristic of the poly(dG)-poly(dG)-poly(dC) triplex. The polymer is resistant to DNase and interacts much more weakly with ethidium bromide as compared with the double-stranded DNA.",
author = "Alexander Kotlyar and Natalia Borovok and Tatiana Molotsky and Dmitry Klinov and Benjamin Dwir and Eli Kapon",
note = "Funding Information: This work is supported by a European Grant for Future & Emerging Technologies (IST-2001-38951). Funding to pay the Open Access publication charges for this article was provided by a European Grant for Future and Emerging Technologies (IST-2001-38951).",
year = "2005",
doi = "10.1093/nar/gki963",
language = "אנגלית",
volume = "33",
pages = "6515--6521",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "20",
}