TY - JOUR
T1 - Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields
AU - Kaufmann, Gabriel
AU - Choder, Mordechai
AU - Groner, Yoram
N1 - Funding Information:
I This work was supported by U S-Israel Science Foundation Grants 1315 to Y.G. G.K: and by a grant from the Israel National of Science to G.K. ’ Incumbent of the Alan Dixon Career ment Chair. ” Incumbent Development
PY - 1980
Y1 - 1980
N2 - The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.
AB - The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.
UR - https://www.scopus.com/pages/publications/0019326240
U2 - 10.1016/0003-2697(80)90029-9
DO - 10.1016/0003-2697(80)90029-9
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AN - SCOPUS:0019326240
SN - 0003-2697
VL - 109
SP - 198
EP - 202
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -